Modifying Enzymes
Biotium CD45 / LCA (Leucocyte Marker)(Bra55), CF594 conjugate, 0.1mg/mL
CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
Worthington Biochemical Corporation BOVINE TRYPSIN DIALYZED AGAIN
Trypsin, 3X Crystallized, Dialyzed Against 1mM HCl and Lyophilized, Store at 2 deg. to 8 deg.C, Source: Bovine Pancreas, Minimum Activity: 180 units/mg Protein, 100mg
Supply Solutions Sigma Aldrich - L-MDH, (pig heart), 25 mg * Third Party Item
Sigma Aldrich - L-MDH, (pig heart), 25 mg * Third Party Item
American Research Products Inc Human deoXyribonuclease I DNa
Human deoXyribonuclease I DNase-I ELISA Kit from CUSABIO Detection Range 1 56 ng/mL-100 ng/mL Sensitivity 0 39 ng/mL Sample serum plasma tissue homogenates Method Sandwich
ABclonal Technology Exonuclease VII
Exonuclease VII (Exo VII) can cleave single-stranded DNA in both 5-3 and 3-5 directions and is inactive against linear or circular double-stranded DNA. When PCR requires stepwise completion to obtain the final target product, this enzyme can be used to remove residual oligonucleotide primers left during intermediate stages of PCR, eliminating interference with subsequent use of different primers. The enzymes digestion of single-stranded DNA is independent of metal ions. This product is a highly purified protein obtained through the recombinant expression of the Exonuclease VII gene (XseA and XseB).
Miltenyi Biotec, Inc. Pancreas TumorMACS Medium
Cell culture medium for the cultivation and expansion of tumor cells from primary and xenotransplanted pancreatic tumors.
This product is subject to the following: Miltenyi Biotec, Inc. Terms and Conditions of Sale For Products and Services
New England Biolabs, Inc. AluI Methyltransferase – 100 units
AluI Methyltransferase modifies the cytosine residue (C5) of the sequence AGCT.
Apexbio Technology LLC DNase I (RNase-free) 10000U
DNase I (RNase-free) is an endodeoxyribonuclease capable of hydrolyzing single-stranded or double-stranded DNA producing dinucleotide trinucleotide and oligonucleotide fragments with 3 -OH and 5 -phosphate ends The activity of DNase I (RNase-free) depends on Ca2 and can be activated by Mg2 or Mn2 In the presence of Mg2 DNase I (RNase-free) randomly cleaves double-stranded DNA at any site in the presence of Mn2 it simultaneously recognizes both DNA strands and cleaves at nearly identical sites DNase I (RNase-free) acts on single-stranded DNA double-stranded DNA chromatin and RNA DNA hybrids In molecular biology experiments DNase I (RNase-free) is suitable for the removal of DNA in RNA extraction in vitro transcription and RT-PCR applications
Qiagen Beverly Inc EXONUCLEASE III
Exonuclease, Exonuclease III; Conc. 100,000 U/ul; 50,000 U; incl. 10X buffer
Apexbio Technology LLC E.-COLI-DNA-LIGASE-1000U
E coli DNA ligase is an enzyme used extensively in molecular biology research particularly within the fields of genetic engineering and biotechnology Originating from the bacterium Escherichia coli this enzyme plays a crucial role in DNA replication and repair by catalyzing the formation of phosphodiester bonds between the 3 -hydroxyl and 5 -phosphate ends of DNA fragments Its mechanism of action involves the joining of Okazaki fragments on the lagging strand during DNA replication as well as sealing nicks in the DNA backbone It exhibits high activity in in vitro applications typically within the nanomolar to low micromolar range E coli DNA ligase is commonly used in applications involving cell models animal studies and drug screening protocols where its ability to ligate DNA fragments is essential Experimental concentrations typically depend on the specific research design allowing for flexibility in various experimental setups
Cayman Chemical 2-Oleoyl Glycerol 5mg
A natural monoacylglycerol that is metabolized by monoacylglycerol lipase; an agonist of GPR119 (EC50 = 2.5 µM), stimulating the release of glucagon-like peptide-1 from intestinal L-cells